Anticorps Monoclonal anti-DLX5

DLX5 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1C9G1

N° de cat : 67111-1-Ig

Synonymes

distal less homeobox 5, DLX5, Homeobox protein DLX 5

---

Galerie de données de validation

• 

  WB analysis using 67111-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 67111-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 67111-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 67111-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 67111-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67111-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67111-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67111-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules PC-12, cellules A375, cellules THP-1, cellules U-251, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67111-1-Ig cible DLX5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DLX5 Protéine recombinante Ag17040
• Nom complet: distal-less homeobox 5
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 33-38 kDa
• Numéro d’acquisition GenBank: BC006226
• Symbole du gène: DLX5
• Identification du gène (NCBI): 1749
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DLX5 belongs to the DLX family of homeobox transcription factors (Dlx1, Dlx2, Dlx5 and Dlx6), which is expressed in overlapping domains at different stages of cell differentiation in the subpallium and controls differentiation of GABAergic neurons. DLX5 is an immediate early BMP-responsive transcriptional activator that essential for osteoblast differentiation. It can bind to and stimulate the homeodomain-response element of the ALPL and SP7 promoter. DLX5 can form homodimer with DLX2(~34kd) and heterodimer with MSX2(~29kd).