Anticorps Monoclonal anti-DLK1

DLK1 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

2C3H8

N° de cat : 67923-1-Ig

Synonymes

DLK, DLK 1, DLK1, FA1, pG2, Pref 1, PREF1, Protein delta homolog 1, ZOG

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Galerie de données de validation

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  WB analysis using 67923-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67923-1-Ig (DLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67923-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67923-1-Ig (DLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig adrenal gland using 67923-1-Ig

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67923-1-Ig (DLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 67923-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67923-1-Ig (DLK1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67923-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67923-1-Ig (DLK1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules JAR, cellules NCCIT, tissu de glande surrénale de porc, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67923-1-Ig cible DLK1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DLK1 Protéine recombinante Ag30916
• Nom complet: delta-like 1 homolog (Drosophila)
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 45-60 kDa
• Numéro d’acquisition GenBank: BC007741
• Symbole du gène: DLK1
• Identification du gène (NCBI): 8788
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DLK1, also named PREF1, FA1, or pG2, is a transmembrane protein belonging to the epidermal growth factor (EGF)-like superfamily (PMID: 8095043). It contains six EGF-like repeats in the extracellular region. DLK1 is abundant in preadipocytes and regulate adipocyte differentiation negatively (PMID: 8500166). Deficiency of DLK1 gives rise to growth retardation and accelerated adiposity in mouse model. Expression of DLK1 is found in tumors with neuroendocrine features that implies DLK1 may be involved in neuroendocrine differentiation (PMID: 8095043). It has been reported overexpression of DLK1 could lead to the development of metabolic abnormalities by impairment of adipocyte function in mice (PMID: 12588883). The gene of DLK1 maps to chromosome 14q32, and encodes a 383-amino acid protein with a calculated molecular mass of 41 kDa. In preadipocytes, multiple discrete forms of DLK1 protein of 45-60 kDa are present, owing in part to N-linked glycosylation (PMID: 8500166).