Anticorps Monoclonal anti-DHX32

• Phare
• Validé par KD/KO

DHX32 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2A4G9

N° de cat : 68306-1-Ig

Synonymes

DDX32, DEAD/H box 32, DEAD/H helicase like protein 1, DEAH box protein 32, DHLP1, DHX32, FLJ10694, FLJ10889, HuDDX32

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Galerie de données de validation

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  WB analysis using 68306-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 68306-1-Ig

  WB result of DHX32 antibody (68306-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX32 transfected A549 cells.

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  WB analysis using 68306-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 68306-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68306-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HeLa, cellules K-562, cellules MCF-7, cellules U2OS, tissu testiculaire de rat, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68306-1-Ig cible DHX32 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DHX32 Protéine recombinante Ag13956
• Nom complet: DEAH (Asp-Glu-Ala-His) box polypeptide 32
• Masse moléculaire calculée: 743 aa, 84 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC002473
• Symbole du gène: DHX32
• Identification du gène (NCBI): 55760
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DHX32, an RNA helicase, belongs to the DEAH box RNA helicase family by virtue of its homology to the conserved helicase domain. DHX32 is abundantly expressed in human and murine models, participating in cell transcription and differentiation, and being capable of regulating T-cell apoptosis. DHX32 is also associated with some malignant tumors, such as lymphoma, colorectal cancer, and breast cancer (PMID: 35117530). DHX32 has 2 isoforms with molecular weights of 75 and 84 kDa, respectively.