Anticorps Monoclonal anti-DGCR8

• Phare
• Validé par KD/KO

DGCR8 Monoclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, RIP, IP, ELISA

Conjugaison

Non conjugué

Publications

9

CloneNo.

4D11A3

N° de cat : 60084-1-Ig

Synonymes

C22orf12, DGCR8, DGCRK6, Gy1

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Galerie de données de validation

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  WB analysis using 60084-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 60084-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 60084-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60084-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 60084-1-Ig

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 60084-1-Ig

  IP Result of anti-DGCR8 (IP:60084-1-Ig, 5ug; Detection:60084-1-Ig 1:500) with K-562 cells lysate 3440ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules BxPC-3, cellules HepG2, cellules Jurkat, cellules K-562, cellules RAW 264.7, cellules ROS1728
• Résultats positifs en IP: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IP: See 1 publications below
• RIP: See 2 publications below

Informations sur le produit

60084-1-Ig cible DGCR8 dans les applications de WB, RIP, IP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DGCR8 Protéine recombinante Ag4871
• Nom complet: DiGeorge syndrome critical region gene 8
• Masse moléculaire calculée: 773 aa, 86 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC009323
• Symbole du gène: DGCR8
• Identification du gène (NCBI): 54487
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.

Publications

Species	Application	Title
mouse,human	WB	Nature; Global miRNA dosage control of embryonic germ layer specification.; Authors - Yingzi Cui ; KO Validated
human	WB	Nat Commun; Hypoxia regulates overall mRNA homeostasis by inducing Met1-linked linear ubiquitination of AGO2 in cancer cells.; Authors - Hailong Zhang
human,rat	WB	Aging Cell; p38 MAPK-mediated loss of nuclear RNase III enzyme Drosha underlies amyloid beta-induced neuronal stress in Alzheimer's disease; Authors - Haidong Xu
human	WB,RIP	Mol Ther; DGCR8/ZFAT-AS1 Promotes CDX2 Transcription in a PRC2 Complex-Dependent Manner to Facilitate the Malignant Biological Behavior of Glioma Cells.; Authors - Fangfang Zhang
human, mouse	WB	Nucleic Acids Res; SUMOylation at K707 of DGCR8 controls direct function of primary microRNA.; Authors - Changhong Zhu
human	WB	Oncogene; Identification of microprocessor-dependent cancer cells allows screening for growth-sustaining micro-RNAs.; Authors - Peric D D