Anticorps Monoclonal anti-DFNA5/GSDME
DFNA5/GSDME Monoclonal Antibody for FC, WB, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, porc, rat, souris
Applications
WB, FC, ELISA
Conjugaison
Non conjugué
CloneNo.
1D9C3
N° de cat : 67731-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | tissu cérébral de porc, cellules HEK-293, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris |
| Résultats positifs en cytométrie | cellules SH-SY5Y, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 3 publications below |
Informations sur le produit
67731-1-Ig cible DFNA5/GSDME dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris
| Réactivité | Humain, porc, rat, souris |
| Réactivité citée | rat, Humain, souris |
| Hôte / Isotype | Mouse / IgG1 |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | DFNA5/GSDME Protéine recombinante Ag30514 |
| Nom complet | deafness, autosomal dominant 5 |
| Masse moléculaire calculée | 496 aa, 55 kDa |
| Poids moléculaire observé | 50-55 kDa |
| Numéro d’acquisition GenBank | BC019689 |
| Symbole du gène | DFNA5 |
| Identification du gène (NCBI) | 1687 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine G |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for DFNA5/GSDME antibody 67731-1-Ig | Download protocol |
| FC protocol for DFNA5/GSDME antibody 67731-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Appl Mater Interfaces Mitochondria-Targeting Pyroptosis Amplifier of Lonidamine-Modified Black Phosphorus Nanosheets for Glioblastoma Treatments | ||
Exp Neurol SNAP25 ameliorates postoperative cognitive dysfunction by facilitating PINK1-dependent mitophagy and impeding caspase-3/GSDME-dependent pyroptosis | ||
ACS Chem Neurosci Inhibition of PINK1-Mediated Mitophagy Contributes to Postoperative Cognitive Dysfunction through Activation of Caspase-3/GSDME-Dependent Pyroptosis |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH William (Verified Customer) (05-12-2021) | This antibody brightly stained bands of just over 50 kDa and just under 30 kDa, neither of which correspond to human GSDME, and it did so in samples from cells that do not express GSDME. The sample blots provided by ProteinTech also show bands that are too small to be GSDME. This does not appear to target GSDME.
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