Anticorps Polyclonal de lapin anti-DENND1A

DENND1A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 25658-1-AP

Synonymes

Connecdenn, DENN/MADD domain containing 1A, DENND1A, FAM31A, KIAA1608, Protein FAM31A, RP11 230L22.3

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Galerie de données de validation

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  WB analysis of SKOV-3 using 25658-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 25658-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25658-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25658-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 25658-1-AP

  IP Result of anti-DENND1A (IP:25658-1-AP, 4ug; Detection:25658-1-AP 1:600) with K-562 cells lysate 3200ug.

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  IHC staining of human endometrial cancer using 25658-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25658-1-AP (DENND1A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 25658-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25658-1-AP (DENND1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 25658-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25658-1-AP (DENND1A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 25658-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25658-1-AP (DENND1A Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 25658-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DENND1A antibody (25658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, cellules A2780, cellules K-562
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

25658-1-AP cible DENND1A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DENND1A Protéine recombinante Ag22547
• Nom complet: DENN/MADD domain containing 1A
• Masse moléculaire calculée: 1009 aa, 111 kDa
• Poids moléculaire observé: 111 kDa, 64 kDa
• Numéro d’acquisition GenBank: BC039703
• Symbole du gène: DENND1A
• Identification du gène (NCBI): 57706
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DENND1A (DENN/MADD domain containing 1A), also known as Connecdenn,FAM31A or KIAA1608, is a 1,009 amino acid peripheral membrane protein. Recent studies has found DENND1A is strongly associated with PCOS (Polycystic ovary syndrome). DENND1A has several isoforms.This antibody(25658-1-AP) can recognize all the isoforms at 112 kDa, 52-64 kDa.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xin (Verified Customer) (03-15-2022)

There is a band close to 130 kD not like the predicted 110 kD around. It is not sure the identity.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human postmortem brain lysate

DENND1A Antibody Western Blot validation (1:1000 dilution) in human postmortem brain lysate (Cat no:25658-1-AP)