Anticorps Monoclonal anti-DDAH2

DDAH2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1A10G3

N° de cat : 68154-1-Ig

Synonymes

DDAH, DDAH 2, DDAH2, DDAHII, Dimethylargininase 2, G6a, NG30, Protein G6a, S phase protein

---

Galerie de données de validation

• 

  WB analysis using 68154-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse retina using 68154-1-Ig

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 68154-1-Ig

  rat colon tissue were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: tissu cérébral de lapin, tissu cérébral de rat, tissu cérébral de souris, tissu de côlon de rat, tissu rétinien de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68154-1-Ig cible DDAH2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DDAH2 Protéine recombinante Ag7127
• Nom complet: dimethylarginine dimethylaminohydrolase 2
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC001435
• Symbole du gène: DDAH2
• Identification du gène (NCBI): 23564
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.