Anticorps Polyclonal de lapin anti-Cytokeratin 8-specific

Cytokeratin 8-specific Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 27105-1-AP

Synonymes

CARD2, Cell and organelle markers, CK 8, CK8, CYK8, Cytokeratin 8, Cytokeratin 8-specific, Cytoskeleton Marker, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8

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Galerie de données de validation

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  WB analysis using 27105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27105-1-AP (Cytokeratin 8 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27105-1-AP (Cytokeratin 8 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skin using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skin using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 27105-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 8-specific antibody (27105-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu cutané de rat, tissu cutané de souris, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

27105-1-AP cible Cytokeratin 8-specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 8-specific Protéine recombinante Ag25844
• Nom complet: keratin 8
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC008200
• Symbole du gène: KRT8
• Identification du gène (NCBI): 3856
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

Publications

Species	Application	Title
human	WB	Transl Lung Cancer Res; Identification of lysosomal genes associated with prognosis in lung adenocarcinoma; Authors - Houqiang Li
human	IHC	Environ Toxicol; Multidimensional progressive single-cell sequencing reveals cell microenvironment composition and cancer heterogeneity in lung cancer; Authors - Hua Chen