Anticorps Monoclonal anti-Cytokeratin 7

• Phare
• Validé par KD/KO

Cytokeratin 7 Monoclonal Antibody for FC(Intra), IF

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IF, FC(Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

2E1G5

N° de cat : CL555-66483

Synonymes

CK 7, CK7, Cytokeratin 7, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

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Galerie de données de validation

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  IF Staining of human prostate cancer using CL555-66483

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®555 Cytokeratin 7 antibody (CL555-66483, Clone: 2E1G5 ) at dilution of 1:200.

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  IF Staining of human prostate cancer using CL555-66483

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®555 Cytokeratin 7 antibody (CL555-66483, Clone: 2E1G5 ) at dilution of 1:200.

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  FC experiment of HeLa using CL555-66483

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug CoraLite®555 Anti-Human Cytokeratin 7 (CL555-66483, Clone:2E1G5) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: tissu de cancer de la prostate humain,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-66483 cible Cytokeratin 7 dans les applications de IF, FC(Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 7 Protéine recombinante Ag7895
• Nom complet: keratin 7
• Masse moléculaire calculée: 469 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC002700
• Symbole du gène: KRT7
• Identification du gène (NCBI): 3855
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named as cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7.

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C