Anticorps Monoclonal anti-Cytokeratin 5

Cytokeratin 5 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

1A1C5

Avis (1)

N° de cat : 66727-1-Ig

Synonymes

58 kDa cytokeratin, Cell and organelle markers, CK 5, CK5, Cytokeratin 5, Cytoskeleton Marker, K5, keratin 5, KRT5, KRT5A, Type II keratin Kb5

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Galerie de données de validation

• 

  WB analysis using 66727-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 66727-1-Ig

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 66727-1-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 66727-1-Ig

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66727-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66727-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 10x lens) showing negative staining. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 40x lens) showing negative staining. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 66727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human oesophagus cancer using 66727-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of A431 using 66727-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human oesophagus cancer using 66727-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human prostate cancer using 66727-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human prostate cancer using 66727-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of A431 using 66727-1-Ig

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 5 (66727-1-Ig, Clone:1A1C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HaCaT, cellules A-253, cellules A431, cellules A549, cellules MCF-7, cellules SKOV-3, salive humain, salive humaine, tissu cutané de porc, tissu cutané de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de la peau humain, tissu de cancer de l'œsophage humain, tissu de cancer du col de l'utérus humain, tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu d'hyperplasie mammaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431, tissu de cancer de la prostate humain, tissu de cancer de l'œsophage humain
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

66727-1-Ig cible Cytokeratin 5 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 5 Protéine recombinante Ag24184
• Nom complet: keratin 5
• Masse moléculaire calculée: 590 aa, 62 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC024292
• Symbole du gène: KRT5
• Identification du gène (NCBI): 3852
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD keratin 14 in stratified squamous epithelia. Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD tyK14 in stratified squamous epithelia.

Publications

Species	Application	Title
rat	IF	Cell Mol Biol Lett; The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis; Authors - Tao Yang
mouse	IHC	Front Genet; The Unique Genetic and Histological Characteristics of DMBA-Induced Mammary Tumors in an Organoid-Based Carcinogenesis Model.; Authors - Mie Naruse
human	WB	PLoS One; Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.; Authors - Kui Xiao
human	WB	Cell Death Dis; SOX1 promotes differentiation of nasopharyngeal carcinoma cells by activating retinoid metabolic pathway.; Authors - Xin-Xing Lei
human	WB	Cell Death Discov; TRIM29 hypermethylation drives esophageal cancer progression via suppression of ZNF750; Authors - Qiyi Yi
mouse	IHC	Immunobiology; High expression of NF-κB inducing kinase in the bulge region of hair follicle induces tumor; Authors - Weisong Duan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Used by IF on FFPE prostate tissue. Tris-EFTA antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Prostate

Cytokeratin 5 Antibody Immunofluorescence validation (1:100 dilution) in Prostate (Cat no:66727-1-Ig)