Anticorps Polyclonal de lapin anti-Cytokeratin 19

Cytokeratin 19 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 29855-1-AP

Synonymes

CK 19, CK19, Cytokeratin 19, K19, K1CS, keratin 19, KRT19

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Galerie de données de validation

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  WB analysis using 29855-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 29855-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 29855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 29855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 29855-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 29855-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 29855-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 29855-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 29855-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 19 antibody (29855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 29855-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Cytokeratin 19 antibody (29855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 29855-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 19 antibody (29855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HepG2, cellules HT-29, cellules MCF-7, cellules T-47D
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la thyroïde humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules A549, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

29855-1-AP cible Cytokeratin 19 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 19 Protéine recombinante Ag31469
• Nom complet: keratin 19
• Masse moléculaire calculée: 44KD
• Poids moléculaire observé: 40-44 kDa
• Numéro d’acquisition GenBank: NM_002276
• Symbole du gène: KRT19
• Identification du gène (NCBI): 3880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.