Anticorps Monoclonal anti-Cytokeratin 18

Cytokeratin 18 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

canin, Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G11C4

N° de cat : CL488-66187

Synonymes

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

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Galerie de données de validation

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  IF Staining of HeLa using CL488-66187

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66187 (Cytokeratin 18 antibody) at dilution of 1:100.

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  IF Staining of MDCK using CL488-66187

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using CL488-66187 (Cytokeratin 18 antibody) at dilution of 1:100.

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  IF Staining of HepG2 using CL488-66187

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66187 (Cytokeratin 18 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using CL488-66187

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66187 (Cytokeratin 18 antibody) at dilution of 1:100.

• 

  FC experiment of HeLa using CL488-66187

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human Cytokeratin 18 (CL488-66187, Clone:1G11C4) (green), and 0.2 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en IF: cellules HeLa, cellules HepG2, cellules MDCK
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66187 cible Cytokeratin 18 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons canin, Humain

• Réactivité: canin, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 18 Protéine recombinante Ag1260
• Nom complet: keratin 18
• Masse moléculaire calculée: 48 kDa
• Numéro d’acquisition GenBank: BC000180
• Symbole du gène: KRT18
• Identification du gène (NCBI): 3875
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.