Anticorps Monoclonal anti-Cystatin B

Cystatin B Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2G9B8

N° de cat : 66812-1-Ig

Synonymes

CPI B, CST6, CSTB, Cystatin B, cystatin B (stefin B), EPM1, PME, Stefin B, STFB

---

Galerie de données de validation

• 

  WB analysis of U-937 using 66812-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human saliva using 66812-1-Ig

  human saliva were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66812-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 66812-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cystatin B antibody (66812-1-Ig, Clone: 2G9B8 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules U-937, cellules THP-1, tissu salivaire humain
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66812-1-Ig cible Cystatin B dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cystatin B Protéine recombinante Ag21291
• Nom complet: cystatin B (stefin B)
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 11-14 kDa
• Numéro d’acquisition GenBank: BC003370
• Symbole du gène: CSTB
• Identification du gène (NCBI): 1476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.