Anticorps Polyclonal de lapin anti-Claudin 5

Claudin 5 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 29767-1-AP

Synonymes

AWAL, BEC1, claudin 5, CLDN5, CPETRL1, TMDVCF, TMVCF

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Galerie de données de validation

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  WB analysis using 29767-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29767-1-AP (Claudin 5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: unboiled mouse lung tissue, unboiled rat lung tissue

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

29767-1-AP cible Claudin 5 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Claudin 5 Protéine recombinante Ag29044
• Nom complet: claudin 5
• Masse moléculaire calculée: 218 aa, 23 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC032363
• Symbole du gène: CLDN5
• Identification du gène (NCBI): 7122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 27 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

Publications

Species	Application	Title
mouse	WB	Life Sci; Metabolomics study reveals increased deoxycholic acid contributes to deoxynivalenol-mediated intestinal barrier injury; Authors - Xin He
rat	WB	Int J Mol Sci; Ursolic Acid Alleviates Neuroinflammation after Intracerebral Hemorrhage by Mediating Microglial Pyroptosis via the NF-κB/NLRP3/GSDMD Pathway; Authors - Pan Lei
mouse	WB	Brain Res Bull; Inhibition of MMP-2 and MMP-9 attenuates surgery-induced cognitive impairment in aged mice; Authors - Yiqin Ji
rat	WB	Mol Biotechnol; Omega-3 Polyunsaturated Fatty Acids Alleviate Intestinal Barrier Dysfunction in Obstructive Jaundice Rats; Authors - Changxi Zhang
mouse	WB	Int Immunopharmacol; Knockdown of Smox protects the integrity of the blood-brain barrier through antioxidant effect and Nrf2 pathway activation in stroke; Authors - Guangtian Wang
rat	WB	Brain Res Bull; PSD-95 inhibitor Tat-NR2B9c (NA-1) protects the integrity of the blood-brain barrier after transient middle artery occlusion in rats by downregulating matrix metalloprotease-9 and upregulating endothelial nitric oxide synthase; Authors - Ye Xu