Anticorps Polyclonal de lapin anti-Claudin 1

Claudin 1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

70

N° de cat : 28674-1-AP

Synonymes

claudin 1, CLD1, CLDN1, ILVASC, SEMP1

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Galerie de données de validation

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  WB analysis using 28674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28674-1-AP (Claudin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28674-1-AP (Claudin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 28674-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Claudin 1 antibody (28674-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HepG2, tissu cutané de rat, tissu cutané de souris
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu cutané de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HUVEC,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 53 publications below
• IHC: See 17 publications below
• IF: See 20 publications below

Informations sur le produit

28674-1-AP cible Claudin 1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Claudin 1 Protéine recombinante Ag29269
• Nom complet: claudin 1
• Masse moléculaire calculée: 211 aa, 23 kDa
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC012471
• Symbole du gène: CLDN1
• Identification du gène (NCBI): 9076
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells.

Publications

Species	Application	Title
	IF	Microbiome; Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O1; Authors - Hao Chen
mouse	WB	J Nanobiotechnology; Human umbilical cord mesenchymal stem cells derived exosome shuttling mir-129-5p attenuates inflammatory bowel disease by inhibiting ferroptosis; Authors - Zhiping Wei
mouse	IF	Matrix Biol; Ameloblastin promotes polarization of ameloblast cell lines in a 3-D cell culture system; Authors - Gayathri Visakan
human,mouse	WB	Biomed Pharmacother; Bacterial butyrate mediates the anti-atherosclerotic effect of silybin; Authors - Hao-Ran Shen
mouse	WB	Biomed Pharmacother; Polysaccharide-rich fractions from Enteromorpha prolifera improve hepatic steatosis and gut barrier integrity in high-fat diet-induced obese mice linking to modulation of gut microbiota; Authors - Tiande Zou
mouse	IF,WB	Oxid Med Cell Longev; Honokiol Ameliorates DSS-Induced Mouse Colitis by Inhibiting Inflammation and Oxidative Stress and Improving the Intestinal Barrier; Authors - Lu Wang