Anticorps Polyclonal de lapin anti-CD31

CD31 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

32

N° de cat : 28083-1-AP

Synonymes

C85791, Cd31, Pecam, PECAM 1, Pecam1

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Galerie de données de validation

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  WB analysis of bEnd.3 using 28083-1-AP

  bEnd.3 cells were subjected to SDS PAGE followed by western blot with 28083-1-AP (CD31 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 28083-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 28083-1-AP (CD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 28083-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 28083-1-AP (CD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 28083-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28083-1-AP (CD31 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28083-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28083-1-AP (CD31 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 28083-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28083-1-AP (Cd31 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 28083-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28083-1-AP (Cd31 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28083-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28083-1-AP (CD31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28083-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28083-1-AP (CD31 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 28083-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CD31 antibody (28083-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse liver using 28083-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CD31 antibody (28083-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules bEnd.3, tissu hépatique de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu hépatique de souris, tissu cérébral de souris, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu hépatique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF): IF : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 13 publications below
• IF: See 15 publications below
• FC: See 1 publications below

Informations sur le produit

28083-1-AP cible CD31 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD31 Protéine recombinante Ag27828
• Nom complet: platelet/endothelial cell adhesion molecule 1
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 110-130 kDa
• Numéro d’acquisition GenBank: NM_001032378
• Symbole du gène: Pecam1
• Identification du gène (NCBI): 18613
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publications

Species	Application	Title
	IHC	Nanomicro Lett; Gelatin-Based Metamaterial Hydrogel Films with High Conformality for Ultra-Soft Tissue Monitoring; Authors - Yuewei Chen
mouse	FC	Cell Mol Biol Lett; Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions; Authors - Seyram Yao Adzraku
mouse	IF	Front Immunol; "γδT Cell-IL17A-Neutrophil" Axis Drives Immunosuppression and Confers Breast Cancer Resistance to High-Dose Anti-VEGFR2 Therapy.; Authors - Zhigang Zhang
mouse	IF	Front Oncol; Hypoxia-Induced LncRNA-MIR210HG Promotes Cancer Progression By Inhibiting HIF-1α Degradation in Ovarian Cancer.; Authors - Ping Liu
mouse	IHC	Front Bioeng Biotechnol; Investigating the Adipogenic Effects of Different Tissue-Derived Decellularized Matrices.; Authors - Weiya Tang
rat		Front Endocrinol (Lausanne); Comparative Transcriptome Analyses of Geriatric Rats Associate Age-Related Erectile Dysfunction With a lncRNA-miRNA-mRNA Regulatory Network.; Authors - Xuan Zhou