Anticorps Monoclonal anti-Cathepsin D

• Phare
• Validé par KD/KO

Cathepsin D Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

7

CloneNo.

2F6F7

N° de cat : 66534-1-Ig

Synonymes

cathepsin D, CLN10, CPSD, CTSD

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Galerie de données de validation

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  WB analysis using 66534-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66534-1-Ig (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66534-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66534-1-Ig (Cathepsin D antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66534-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66534-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66534-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66534-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66534-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66534-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66534-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cathepsin D antibody (66534-1-Ig, Clone: 2F6F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66534-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Cathepsin D (66534-1-Ig, Clone:2F6F7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: Saos-2 cells, cellules HepG2, cellules MCF-7, cellules T-47D
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

66534-1-Ig cible Cathepsin D dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cathepsin D Protéine recombinante Ag15254
• Nom complet: cathepsin D
• Masse moléculaire calculée: 412 aa, 45 kDa
• Poids moléculaire observé: 32 kDa, 48 kDa, 52 kDa
• Numéro d’acquisition GenBank: BC016320
• Symbole du gène: CTSD
• Identification du gène (NCBI): 1509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTSD (Cathepsin D) is also named as CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. (PMID: 27114232, PMID: 30717773, PMID: 30051532)

Publications

Species	Application	Title
human	IHC	Gastroenterology; Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors; Authors - Mingjun Sun
rat	WB,IF	Life (Basel); Melibiose Confers a Neuroprotection against Cerebral Ischemia/Reperfusion Injury by Ameliorating Autophagy Flux via Facilitation of TFEB Nuclear Translocation in Neurons.; Authors - Zhiyuan Wu
	WB	Cell Rep; Intracellular complement C5a/C5aR1 stabilizes β-catenin to promote colorectal tumorigenesis.; Authors - Peipei Ding ; KD Validated
human	IF	FEBS Lett; Senile plaques in Alzheimer's disease arise from Aβ- and Cathepsin D-enriched mixtures leaking out during intravascular haemolysis and microaneurysm rupture; Authors - Hualin Fu
human,rat	WB	Ecotoxicol Environ Saf; The inhibition of TRPML1/TFEB leads to lysosomal biogenesis disorder, contributes to developmental fluoride neurotoxicity; Authors - Jingjing Zhang
rat	WB	Ecotoxicol Environ Saf; TFE3-mediated impairment of lysosomal biogenesis and defective autophagy contribute to fluoride-induced hepatotoxicity; Authors - Zeyu Hu