Anticorps Polyclonal de lapin anti-Caspase 7/p20

• Phare
• Validé par KD/KO

Caspase 7/p20 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

49

N° de cat : 27155-1-AP

Synonymes

Apoptotic protease Mch 3, CASP 7, CASP7, Caspase 7, Caspase 7/p20, Caspase7, CMH 1, ICE LAP3, ICE like apoptotic protease 3, MCH3

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Galerie de données de validation

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  WB analysis using 27155-1-AP

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 27155-1-AP

  WB result of Caspase 7 antibody (27155-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 7 transfected HEK-293 cells.

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  WB analysis of LNCaP using 27155-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 27155-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 27155-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 27155-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 27155-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 27155-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 27155-1-AP

  IP result of anti-Caspase 7 (IP:27155-1-AP, 4ug; Detection:27155-1-AP 1:1000) with HEK-293 cells lysate 2600 ug.

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  IF Staining of MCF-7 using 27155-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 27155-1-AP (Caspase 7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 27155-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Caspase 7/p20 antibody (27155-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 27155-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Caspase 7/p20 antibody (27155-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules LNCaP, cellules MCF-7, cellules PC-3, tissu hépatique de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IF: cellules MCF-7, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 47 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

27155-1-AP cible Caspase 7/p20 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Caspase 7/p20 Protéine recombinante Ag25904
• Nom complet: caspase 7, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 303 aa, 34 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC015799
• Symbole du gène: CASP7
• Identification du gène (NCBI): 840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.

Publications

Species	Application	Title
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	J Pineal Res; Melatonin synergizes the chemotherapeutic effect of 5-fluorouracil in colon cancer by suppressing PI3K/AKT and NF-κB/iNOS signaling pathways.; Authors - Yue Gao
human	WB	Cell Rep; Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation; Authors - Zehe Ge
human	WB	Front Immunol; Identification of molecular subtypes based on PANoptosis-related genes and construction of a signature for predicting the prognosis and response to immunotherapy response in hepatocellular carcinoma; Authors - Jinfeng Zhu
human	WB	Phytother Res; Targeting HSP90 with picropodophyllin suppresses gastric cancer tumorigenesis by disrupting the association of HSP90 and AKT; Authors - Xiaoli Li
human	WB	J Agric Food Chem; β-Sitosterol-D-glucoside from sweet potato exerts an anti-breast cancer activity by upregulating microRNA-10a and PI3K/Akt signaling pathway.; Authors - Heshan Xu