Anticorps Monoclonal anti-Cardiac Troponin I

Cardiac Troponin I Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ISFET, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

CloneNo.

1D5D6

Avis (1)

N° de cat : 66376-1-Ig

Synonymes

Cardiac troponin I, CMD2A, CMH7, cTnI, RCM1, TNNC1, TNNI3, troponin I type 3 (cardiac), Troponin I, cardiac muscle

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Galerie de données de validation

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  WB analysis using 66376-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66376-1-Ig

  Human heart tissue and skeletal muscle tissue (negative control) were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:20,000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 66376-1-Ig

  Mouse heart tissue (6 ug/lane) was subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:5,000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 66376-1-Ig

  Rat heart tissue were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin Iantibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 66376-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 10x lens).

• 

  IHC staining of human heart using 66376-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 40x lens).

• 

  IHC staining of mouse heart using 66376-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 66376-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 66376-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin I antibody (66376-1-Ig, Clone: 1D5D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse heart using 66376-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed mouse heart tissue using 66376-1-Ig(Cardiac Troponin I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse heart using 66376-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed mouse heart tissue using 66376-1-Ig(Cardiac Troponin I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, tissu cardiaque de lapin, tissu cardiaque de porc, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cardiaque de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 6 publications below

Informations sur le produit

66376-1-Ig cible Cardiac Troponin I dans les applications de WB, ISFET, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cardiac Troponin I Protéine recombinante Ag16758
• Nom complet: troponin I type 3 (cardiac)
• Masse moléculaire calculée: 210 aa, 24 kDa
• Poids moléculaire observé: 26-28 kDa
• Numéro d’acquisition GenBank: BC096165
• Symbole du gène: TNNI3
• Identification du gène (NCBI): 7137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.

Publications

Species	Application	Title
human	ISFET	Biosens Bioelectron; A handheld testing device for the fast and ultrasensitive recognition of cardiac troponin I via an ion-sensitive field-effect transistor; Authors - Yiqing Wang
pig	WB	Stem Cell Reports; Physiological calcium combined with electrical pacing accelerates maturation of human engineered heart tissue; Authors - Shi Shen
human	IF	Stem Cell Res Ther; Intravenously transplanted mesenchymal stromal cells: a new endocrine reservoir for cardioprotection.; Authors - Anan Huang
mouse	IF	J Am Heart Assoc; Cardiomyocyte-Specific Snrk Prevents Inflammation in the Heart.; Authors - Karthikeyan Thirugnanam
mouse	WB	J Cell Mol Med; Scoparone alleviates Ang II-induced pathological myocardial hypertrophy in mice by inhibiting oxidative stress.; Authors - Linmao Lyu
mouse	IF	Front Pharmacol; Setanaxib (GKT137831) Ameliorates Doxorubicin-Induced Cardiotoxicity by Inhibiting the NOX1/NOX4/Reactive Oxygen Species/MAPK Pathway.; Authors - Hui Zheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

KARTHIKEYAN (Verified Customer) (11-20-2019)

We used this antibody to stain HL-1 cardiomyocyte cells, and the antibody works really well.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100 for IF
• Cell Tissue Type: Moue HL-1 Atrial cardiomyocytes

Cardiac Troponin I Antibody Immunofluorescence, validation (1:100 for IF dilution) in Moue HL-1 Atrial cardiomyocytes (Cat no:66376-1-Ig)