Anticorps Monoclonal anti-Calretinin

Calretinin Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

CloneNo.

2D7A9

N° de cat : 66496-1-Ig

Synonymes

29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR

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Galerie de données de validation

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  WB analysis using 66496-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis of rat cerebellum using 66496-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 66496-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66496-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66496-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66496-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 66496-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 66496-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 66496-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human appendicitis using 66496-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human appendicitis using 66496-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, cellules U-251, cellules U2OS, tissu cérébral de lapin, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu d'appendicite humain, tissu cérébral de rat, tissu cérébral humain, tissu de cervelet humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y, tissu d'appendicite humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 6 publications below

Informations sur le produit

66496-1-Ig cible Calretinin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Calretinin Protéine recombinante Ag2924
• Nom complet: calbindin 2
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC015484
• Symbole du gène: CALB2
• Identification du gène (NCBI): 794
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

Publications

Species	Application	Title
mouse	IF	Cell; Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.; Authors - Haibo Zhou
mouse	WB,IF	Acta Neuropathol Commun; TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss.; Authors - Tuancheng Feng
mouse	WB,IF	Neurobiol Dis; Sex specific correlation between GABAergic disruption in the dorsal hippocampus and flurothyl seizure susceptibility after neonatal hypoxic-ischemic brain injury.; Authors - Charles R Lechner
mouse	IF	Neurobiol Dis; Cytohesin-2 mediates group I metabotropic glutamate receptor-dependent mechanical allodynia through the activation of ADP ribosylation factor 6 in the spinal cord.; Authors - Akiko Ito
human	WB	J Cancer; A Prognostic Model of Angiogenesis and Neutrophil Extracellular Traps Related Genes Manipulating Tumor Microenvironment in Colon Cancer; Authors - Dongsheng Zhang
mouse	IF	Invest Ophthalmol Vis Sci; Topical Ripasudil Suppresses Retinal Ganglion Cell Death in a Mouse Model of Normal Tension Glaucoma.; Authors - Kei Akaiwa