Anticorps Polyclonal de lapin anti-Calpain 7

• Phare
• Validé par KD/KO

Calpain 7 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 26985-1-AP

Synonymes

calpain 7, CALPAIN7, CAPN7, PalB homolog, PALBH

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Galerie de données de validation

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  WB analysis of mouse brain using 26985-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26985-1-AP (Calpain 7 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26985-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26985-1-AP (Calpain 7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26985-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26985-1-AP (Calpain 7 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, tissu cérébral de rat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

26985-1-AP cible Calpain 7 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Calpain 7 Protéine recombinante Ag25574
• Nom complet: calpain 7
• Masse moléculaire calculée: 93 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: BC056202
• Symbole du gène: CAPN7
• Identification du gène (NCBI): 23473
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain 7 (also known as PalBH) is a mammalian homologue of the Aspergillus, atypical calpain PalB. It is a unique intracellular cysteine protease that has a tandem repeat of microtubule interacting and trafficking (MIT) domains and lacks a penta-EF-hand domain.

Publications

Species	Application	Title
mouse	WB	EMBO Mol Med; CRTH2 promotes endoplasmic reticulum stress-induced cardiomyocyte apoptosis through m-calpain.; Authors - Shengkai Zuo
human	WB,IF	Elife; Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission; Authors - Dawn M Wenzel
human	WB,IF	Elife; The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission; Authors - Elliott L Paine ; KD Validated