Anticorps Polyclonal de lapin anti-CYTSA

CYTSA Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 25390-1-AP

Synonymes

cytospin A, CYTSA, KIAA0376, SPECC1 like protein, SPECC1L

---

Galerie de données de validation

• 

  WB analysis of NIH/3T3 using 25390-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25390-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25390-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 25390-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 25390-1-AP

  IP Result of anti-CYTSA (IP:25390-1-AP, 4ug; Detection:25390-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 25390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25390-1-AP (CYTSA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25390-1-AP (CYTSA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HeLa, cellules Neuro-2a, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

25390-1-AP cible CYTSA dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYTSA Protéine recombinante Ag21896
• Nom complet: cytospin A
• Masse moléculaire calculée: 1117 aa, 125 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC136471
• Symbole du gène: CYTSA
• Identification du gène (NCBI): 23384
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYTSA, also named as SPECC1L or KIAA0376, is a 1117 amino acid protein, which contains one CH domain and belongs to the cytospin-A family. CYTSA localizes in the cytoplasm and is involved in cytokinesis and spindle organization. CYTSA may play a role in actin cytoskeleton organization and microtubule stabilization, hence it is required for proper cell adhesion and migration.

Publications

Species	Application	Title
mouse	WB,IF	Hum Mol Genet; In-frame deletion of SPECC1L microtubule association domain results in gain-of-function phenotypes affecting embryonic tissue movement and fusion events.; Authors - Jeremy P Goering
human	WB,IF	Hum Mol Genet; SPECC1L regulates palate development downstream of IRF6.; Authors - Everett G Hall
human	WB	Curr Biol; EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules; Authors - Takashi Hotta
human	WB,IF,IP	J Biol Chem; SPECC1L binds the myosin phosphatase complex MYPT1/PP1β and can regulate its distribution between microtubules and filamentous actin; Authors - Virja Mehta