Anticorps Polyclonal de lapin anti-CYTB

CYTB Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

75

Avis (1)

N° de cat : 55090-1-AP

Synonymes

COB, Complex III subunit 3, Complex III subunit III, CYTB, cytochrome b, MT CYB, MTCYB, MT-CYTB

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Galerie de données de validation

• 

  WB analysis using 55090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55090-1-AP (CYTB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules MDA-MB-231, cellules T-47D

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 74 publications below
• IF: See 1 publications below

Informations sur le produit

55090-1-AP cible CYTB dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cytochrome b
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: YP_003024038
• Symbole du gène: CYTB
• Identification du gène (NCBI): 4519
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYTB, also named as COB and MTCYB, is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. It's mitochondrion membrane protein with MW 43 kDa.

Publications

Species	Application	Title
human	WB	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang
human	WB	Cell Rep Med; End binding-3 inhibitor activates regenerative program in age-related macular degeneration; Authors - Quinn Lee
human	WB	Nat Commun; RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.; Authors - Uwe Richter
mouse	WB	Nat Commun; Mitofusin 1 and 2 regulation of mitochondrial DNA content is a critical determinant of glucose homeostasis.; Authors - Vaibhav Sidarala
human	WB	Nat Commun; Mitochondrial peptide BRAWNIN is essential for vertebrate respiratory complex III assembly.; Authors - Shan Zhang
human	WB	Nucleic Acids Res; A coronary artery disease-associated tRNAThr mutation altered mitochondrial function, apoptosis and angiogenesis.; Authors - Zidong Jia

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (02-10-2023)

Works very well in differentiated human white adipocyte cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: adipocytes