Anticorps Monoclonal anti-CYP3A4

CYP3A4 Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(1)

CloneNo.

2F11C4

N° de cat : CL488-67110

Synonymes

CYP3A3, CYP3A, CP34, CP33, Cholesterol 25-hydroxylase

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Galerie de données de validation

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  IF Staining of HepG2 using CL488-67110

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 CYP3A4 antibody (CL488-67110, Clone: 2F11C4 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL488-67110

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 CYP3A4 antibody (CL488-67110, Clone: 2F11C4 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-67110

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CYP3A4 (CL488-67110, Clone:2F11C4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL488-67110 cible CYP3A4 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CYP3A4 Protéine recombinante Ag13074
• Nom complet: cytochrome P450, family 3, subfamily A, polypeptide 4
• Masse moléculaire calculée: 503 aa, 57 kDa
• Poids moléculaire observé: 51-57 kDa
• Numéro d’acquisition GenBank: BC101631
• Symbole du gène: CYP3A4
• Identification du gène (NCBI): 1576
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytochrome P450 3A(CYP3A) belongs to the cytochrome P450 family and the enzymes constitute an important detoxification system that contributes to primary metabolism of more than half of all prescribed medications.CYP3A expression determines impairment of drug absorption and efficient systemic clearance in a tissue-specific manner(PMID:17975676). The murine homolog of human CYP3A4 is CYP3A11 (PMID: 19297428). The highest amounts of drug-metabolizing cytochromes P-450 are found in the liver and intestine, with lower amounts found in other organs, including the kidney, lung, and brain. LPS that has reached the circulation stimulates a TLR4-dependent mechanism in the periphery, evoking a reduction in Cyp3a11 expression and metabolism in the liver (PMID: 15878984).

Publications

Species	Application	Title
human	IF	ACS Appl Bio Mater; 3D Bioprinting of Sugar Beet Pectin through Horseradish Peroxidase-Catalyzed Cross-Linking; Authors - Wildan Mubarok

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