Anticorps Monoclonal anti-CYP11A1

• Phare
• Validé par KD/KO

CYP11A1 Monoclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

1C5D5

N° de cat : 67264-1-Ig

Synonymes

Cholesterol desmolase, CYP11A, CYP11A1, CYPXIA1, Cytochrome P450 11A1, Cytochrome P450(scc), P450SCC

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 67264-1-Ig

  WB result of CYP11A1 antibody (67264-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP11A1 transfected HepG2 cells.

• 

  WB analysis of human placenta using 67264-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67264-1-Ig (CYP11A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67264-1-Ig

  WB result of CYP11A1 antibody (67264-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP11A1 transfected HepG2 cells.

• 

  WB analysis of HepG2 using 67264-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67264-1-Ig (CYP11A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 67264-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67264-1-Ig (CYP11A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 67264-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67264-1-Ig (CYP11A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 67264-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67264-1-Ig (CYP11A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules Caco-2, cellules HepG2
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

67264-1-Ig cible CYP11A1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CYP11A1 Protéine recombinante Ag4353
• Nom complet: cytochrome P450, family 11, subfamily A, polypeptide 1
• Masse moléculaire calculée: 521 aa, 60 kDa
• Poids moléculaire observé: 49-52 kDa
• Numéro d’acquisition GenBank: BC032329
• Symbole du gène: CYP11A1
• Identification du gène (NCBI): 1583
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse,rat	WB	Front Neurosci; Autism-Like Behavior in the Offspring of CYP11A1-Overexpressing Pregnant Rats.; Authors - Tianying Pan
human	IHC	Reprod Sci; Identification of Key Differentially Methylated/Expressed Genes and Pathways for Ovarian Endometriosis by Bioinformatics Analysis; Authors - Haibo Zhang
		Cell Rep; Dissecting the spermatogonial stem cell niche using spatial transcriptomics; Authors - Shreya Rajachandran
mouse	IHC,WB	Toxicol Appl Pharmacol; Reproductive effects of pubertal exposure to neonicotinoid thiacloprid in immature male mice; Authors - Yong Zou
human	WB	Mol Hum Reprod; Reduced oxygen concentrations regulate the phenotype and function of human granulosa cells in vitro and cause a diminished steroidogenic but increased inflammatory cellular reaction; Authors - Maria Höfner