Anticorps Monoclonal anti-CXCL16

• Phare
• Validé par KD/KO

CXCL16 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

2H7B3

N° de cat : 60123-1-Ig

Synonymes

C X C motif chemokine 16, CXCL16, CXCLG16, SCYB16, Small inducible cytokine B16, SR PSOX, SRPSOX, Transmembrane chemokine CXCL16

---

Galerie de données de validation

• 

  WB analysis of SH-SY5Y using 60123-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60123-1-Ig (CXCL16 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

60123-1-Ig cible CXCL16 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CXCL16 Protéine recombinante Ag4883
• Nom complet: chemokine (C-X-C motif) ligand 16
• Masse moléculaire calculée: 273 aa, 30 kDa
• Poids moléculaire observé: 35 kDa, 60 kDa
• Numéro d’acquisition GenBank: BC017588
• Symbole du gène: CXCL16
• Identification du gène (NCBI): 58191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CXCL16 is a recently discovered cytokine belonging to the CXC chemokine family, which is synthesised in plasmacytoid dendritic cell as a transmembrane molecule. It exists in a transmembrane and soluble form. The transmembrane form of CXCL16 functions as an adhesion molecule for CXCR6-expressing cells, whereas the soluble form of CXCL16 mediates infiltration of circulating cells into sites of injury. CXCL16, has been proposed as an important pathogenic mediator in inflammatory diseases, including rheumatoid arthritis, glomerulonephritis, or prostate cancer. CXCL16 has been implicated in some forms of renal disease such as lupus nephritis and antiglomerular basement membrane nephritis. CXCL16 also plays a pivotal role in the pathogenesis of angiotensin II-induced renal injury and fibrosis through regulation of macrophage and T cell infiltration and bone marrow-derived fibroblast accumulation.

Publications

Species	Application	Title
rat	IHC	Cancer Commun (Lond); Activation of Piezo1 contributes to matrix stiffness-induced angiogenesis in hepatocellular carcinoma; Authors - Miao Li
mouse	WB	FASEB J; Pathogenic Tconvs promote inflammatory macrophage polarization through GM-CSF and exacerbate abdominal aortic aneurysm formation.; Authors - Dan Li
mouse	WB	Mol Med Rep; oxLDL promotes podocyte migration by regulating CXCL16, ADAM10 and ACTN4.; Authors - Yuan Chen ; KO Validated
human,mouse	IF	Cardiovasc Diabetol; Klotho inhibits renal ox-LDL deposition via IGF-1R/RAC1/OLR1 signaling to ameliorate podocyte injury in diabetic kidney disease; Authors - Wei Jiang