Anticorps Monoclonal anti-Alpha E-Catenin

Alpha E-Catenin Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

2B7B8

N° de cat : 66221-1-Ig

Synonymes

Alpha E catenin, Alpha E-Catenin, Cadherin associated protein, CAP102, Catenin alpha 1, CTNNA1

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 66221-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66221-1-Ig (Alpha E catenin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 66221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 66221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 66221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 66221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 40x lens).

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  FC experiment of MCF-7 using 66221-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Alpha E-Catenin (66221-1-Ig, Clone:2B7B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3
• Résultats positifs en IHC: tissu d'estomac humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:1000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

66221-1-Ig cible Alpha E-Catenin dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Alpha E-Catenin Protéine recombinante Ag23603
• Nom complet: catenin (cadherin-associated protein), alpha 1, 102kDa
• Masse moléculaire calculée: 906 aa, 100 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: BC031262
• Symbole du gène: CTNNA1
• Identification du gène (NCBI): 1495
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.

Publications

Species	Application	Title
mouse	IHC	Int J Mol Sci; Histological and Immunohistochemical Studies to Determine the Mechanism of Cleft Palate Induction after Palatal Fusion in Mice Exposed to TCDD.; Authors - Chisato Sakuma
human	WB	Oncotarget; YAP1 enhances cell proliferation, migration, and invasion of gastric cancer in vitro and in vivo.; Authors - Dan Sun
human	WB,IHC	Mol Med Rep; Wild-type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β-catenin pathway; Authors - Xianhua Hu