Anticorps Polyclonal de lapin anti-CSNK1A1

• Phare
• Validé par KD/KO

CSNK1A1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 55192-1-AP

Synonymes

casein kinase 1, alpha 1, Casein kinase I isoform alpha, CK1, CKI alpha, CSNK1A1, HLCDGP1, PRO2975

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Galerie de données de validation

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  WB analysis using 55192-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55192-1-AP (CSNK1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 55192-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55192-1-AP (CSNK1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 55192-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55192-1-AP (CSNK1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55192-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55192-1-AP (CSNK1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 55192-1-AP

  Immunofluorescent analysis of A549 cells using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 55192-1-AP

  Immunofluorescent analysis of A549 cells using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

55192-1-AP cible CSNK1A1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: casein kinase 1, alpha 1
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 30-35 kDa, 42-45 kDa
• Numéro d’acquisition GenBank: NM_001892
• Symbole du gène: CSNK1A1
• Identification du gène (NCBI): 1452
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CSNK1A1, also named as CK1, belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and Casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. CSNK1A1 phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. This antibody is specific to CSNK1A1.

Publications

Species	Application	Title
human	WB,IHC	Pharmacol Res; Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex; Authors - Li Gu ; KD Validated
	WB	J Cell Biol; CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.; Authors - Xue Deng
human	IP	Virol Sin; CK1α upregulates the IFNAR1 expression to prompt the anti-HBV effect of type I IFN in hepatoma carcinoma cells; Authors - Jing Xiong
mouse	WB,IF	Stem Cell Res Ther; Nicotinamide improves in vitro lens regeneration in a mouse capsular bag model.; Authors - Xiaomin Liu
human	WB	Int J Biol Sci; Pantothenate Kinase 1 Inhibits the Progression of Hepatocellular Carcinoma by Negatively Regulating Wnt/β-catenin Signaling.; Authors - Yuyuan Zi
mouse	WB,IF	Endocrinology; Casein kinase 1α regulates testosterone synthesis and testis development in adult mice; Authors - Hongzhou Guo