Anticorps Polyclonal de lapin anti-CRLS1

CRLS1 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 51055-1-AP

Synonymes

C20orf155, Cardiolipin synthase, cardiolipin synthase 1, CLS, CLS1, CRLS1, GCD10, Protein GCD10 homolog

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Galerie de données de validation

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  WB analysis of mouse heart using 51055-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 51055-1-AP (CRLS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 51055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 51055-1-AP (CRLS1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 51055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 51055-1-AP (CRLS1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse heart using 51055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 51055-1-AP (CRLS1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse heart using 51055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 51055-1-AP (CRLS1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 51055-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51055-1-AP (CRLS1 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu cardiaque de souris, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

51055-1-AP cible CRLS1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cardiolipin synthase 1
• Masse moléculaire calculée: 301 aa, 32 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC069010
• Symbole du gène: CRLS1
• Identification du gène (NCBI): 54675
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cardiolipin (CL) is a mitochondrial specific double negatively charged phospholipid which is intricately involved in regulating bioenergetic efficiency. In eukaryotes, CL is synthesized by cardiolipin synthase (CRLS1 or CLS1) which catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form CL and glycerol. Recently it has been reported that transgenic expression of CRLS1 accelerates cardiolipin remodeling, improves mitochondrial function, modulates mitochondrial signaling, and attenuates mitochondrial dysfunction during diabetes, thereby identifying CRLS1 as a novel therapeutic target to attenuate mitochondrial dysfunction in diabetic myocardium. The predicted molecular weight of CRLS1 is around 32-35 kDa, while a 50 kDa protein with CRLS1 activity had been observed in liver isolation, which may represent an isoform of CRLS1 (20652826).

Publications

Species	Application	Title
mouse	WB	Nat Commun; Lipocalin 2 regulates mitochondrial phospholipidome remodeling, dynamics, and function in brown adipose tissue in male mice; Authors - Hongming Su
mouse	WB	Hepatology; Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury; Authors - Li Chen ; KD Validated