Anticorps Polyclonal de lapin anti-CDK12/CRKRS

• Phare
• Validé par KD/KO

CDK12/CRKRS Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 26816-1-AP

Synonymes

CDC2 related protein kinase 7, CDK12, CDK12/CRKRS, CRK7, CRKR, CRKRS, KIAA0904

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Galerie de données de validation

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  WB analysis of HeLa using 26816-1-AP

  WB result of CDK12/CRKRS antibody (26816-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK12/CRKRS transfected HeLa cells.

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  WB analysis using 26816-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26816-1-AP (CDK12/CRKRS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 26816-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26816-1-AP (CRKRS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 26816-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26816-1-AP (CRKRS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules K-562
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

26816-1-AP cible CDK12/CRKRS dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDK12/CRKRS Protéine recombinante Ag25323
• Nom complet: Cdc2-related kinase, arginine/serine-rich
• Masse moléculaire calculée: 1490 aa, 164 kDa
• Poids moléculaire observé: 180-200 kDa
• Numéro d’acquisition GenBank: BC150265
• Symbole du gène: CRKRS
• Identification du gène (NCBI): 51755
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CRKRS (Cdc2-related kinase, Arg/Ser), also named as cyclin-dependent kinase 12 (CKD12), belongs to CDC2/CDKX subfamily. CDK12 can form a heterodimeric complex with its activating partner, cyclin K, to regulate important cellular physiological processes that include transcriptional regulation, regulation of DNA damage repair genes such as BRCA1 and ATM, suppression of intronic polyadenylation, and susceptibility to PARP inhibitors (PMID: 30487607, 24240700, 31694772). CDK12 has 3 isoforms with the calculated molecular mass of 164/163/141 kDa, and the apparent molecular mass of 180-200 kDa due to some modifications (PMID 11683387, 22988298).

Publications

Species	Application	Title
human	WB	Eur J Cancer; CDK12 inhibition enhances sensitivity of HER2+ breast cancers to HER2-tyrosine kinase inhibitor via suppressing PI3K/AKT.; Authors - Hui Li
human	WB	iScience; THOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rate; Authors - Mareike Polenkowski
human		Int J Med Sci; Expression pattern of CDK12 protein in gastric cancer and its positive correlation with CD8+ cell density and CCL12 expression.; Authors - Jun Ji
human	WB	Cell Death Discov; JWA suppresses proliferation in trastuzumab-resistant breast cancer by downregulating CDK12.; Authors - Yan Liang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

M (Verified Customer) (06-29-2021)

No Signal at all at the right size. A couple of non specific bands showed up at lower molecular weights.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver lysate

CDK12/CRKRS Antibody Western Blot validation (1:1000 dilution) in Human Liver lysate (Cat no:26816-1-AP)