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Anticorps Monoclonal anti-CREB3L1,OASIS

CREB3L1,OASIS Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B5B4

N° de cat : CL488-67617

Synonymes

CREB3L1, OASIS

Galerie de données de validation

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated CREB3L1,OASIS Monocl (CL488-67617)

    FC experiment of HepG2 using CL488-67617

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CREB3L1,OASIS (CL488-67617, Clone:2B5B4) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en cytométriecellules HepG2

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-67617 cible CREB3L1,OASIS dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène CREB3L1,OASIS Protéine recombinante Ag28042
Nom complet cAMP responsive element binding protein 3-like 1
Masse moléculaire calculée 57 kDa
Poids moléculaire observé 58 kDa
Numéro d’acquisition GenBankBC015781
Symbole du gène CREB3L1
Identification du gène (NCBI) 90993
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)

Protocole

Product Specific Protocols
FC protocol for CL Plus 488 CREB3L1,OASIS antibody CL488-67617Download protocol
Standard Protocols
Click here to view our Standard Protocols