- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-CREB1
CREB1 Polyclonal Antibody for FC (Intra), IF
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, singe, souris
Applications
IF, FC (Intra)
Conjugaison
CoraLite® Plus 488 Fluorescent Dye
N° de cat : CL488-12208
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en IF | cellules HeLa, |
Résultats positifs en cytométrie | cellules HeLa |
Dilution recommandée
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
CL488-12208 cible CREB1 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, singe, souris
Réactivité | Humain, rat, singe, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | CREB1 Protéine recombinante Ag2852 |
Nom complet | cAMP responsive element binding protein 1 |
Masse moléculaire calculée | 341 aa, 35 kDa |
Poids moléculaire observé | 43-46 kDa |
Numéro d’acquisition GenBank | BC010636 |
Symbole du gène | CREB1 |
Identification du gène (NCBI) | 1385 |
Conjugaison | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3. |
Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).
Protocole
Product Specific Protocols | |
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IF protocol for CL Plus 488 CREB1 antibody CL488-12208 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |