Anticorps Polyclonal de lapin anti-CPT2

CPT2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

24

Avis (1)

N° de cat : 26555-1-AP

Synonymes

CPT II, CPT1, CPT2, CPTASE

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Galerie de données de validation

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  WB analysis using 26555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26555-1-AP (CPT2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26555-1-AP (CPT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 26555-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26555-1-AP (CPT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 26555-1-AP

  IP Result of anti-CPT2 (IP:26555-1-AP, 4ug; Detection:26555-1-AP 1:300) with mouse liver tissue lysate 6000ug.

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  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of C2C12 using 26555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using 26555-1-AP (CPT2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules LNCaP, cellules MCF-7, cellules T-47D, tissu cardiaque de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 22 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 4 publications below

Informations sur le produit

26555-1-AP cible CPT2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPT2 Protéine recombinante Ag24897
• Nom complet: carnitine palmitoyltransferase 2
• Masse moléculaire calculée: 74 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC002445
• Symbole du gène: CPT2
• Identification du gène (NCBI): 1376
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB,IP	Cell Stem Cell; A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.; Authors - Jianyong Du
mouse	WB	Nat Commun; Hyodeoxycholic acid ameliorates nonalcoholic fatty liver disease by inhibiting RAN-mediated PPARα nucleus-cytoplasm shuttling; Authors - Jing Zhong
mouse	WB	Theranostics; Mitochondrial STAT3 exacerbates LPS-induced sepsis by driving CPT1a-mediated fatty acid oxidation.; Authors - Rongqing Li
mouse	WB,IP	Elife; Remodeling of whole-body lipid metabolism and a diabetic-like phenotype caused by loss of CDK1 and hepatocyte division.; Authors - Jin Rong Ow
mouse	WB	J Nanobiotechnology; Regulating immune memory and reversing tumor thermotolerance through a step-by-step starving-photothermal therapy; Authors - Lihua Luo
mouse	WB	Commun Biol; Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism; Authors - Michael Landowski

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mitchell (Verified Customer) (09-26-2019)

Worked great for western blot in proximal intestine and liver.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Intestine and Liver