Anticorps Polyclonal de lapin anti-CPA4

CPA4 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 26824-1-AP

Synonymes

Carboxypeptidase A3, carboxypeptidase A4, CPA3, CPA4

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Galerie de données de validation

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  WB analysis of mouse heart using 26824-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26824-1-AP (CPA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 26824-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26824-1-AP (CPA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 26824-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26824-1-AP (CPA4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 26824-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 26824-1-AP (CPA4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 26824-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26824-1-AP (CPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 26824-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26824-1-AP (CPA4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26824-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26824-1-AP (CPA4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules A549, cellules HepG2, cellules SMMC-7721, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

26824-1-AP cible CPA4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPA4 Protéine recombinante Ag25327
• Nom complet: carboxypeptidase A4
• Masse moléculaire calculée: 421 aa, 47 kDa
• Poids moléculaire observé: 44 kDa, 150 kDa
• Numéro d’acquisition GenBank: BC052289
• Symbole du gène: CPA4
• Identification du gène (NCBI): 51200
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Carboxypeptidase A4 (CPA4), a member of the metallocarboxypeptidase family, is a zinc-containing exopeptidase that catalyzes the release of carboxy-terminal amino acids. CPA4 was previously shown to play a crucial role in cell growth and differentiation by modulating or inactivating peptide hormone activity (PMID: 27904708). CPA4 expression is increased in multiple cancer tissues, including tissues from patients with pancreatic cancer, gastric cancer, esophageal squamous cell carcinoma, and lung cancer, and may serve as a potential diagnostic and prognostic marker (PMID: 31397502, 32922037).

Publications

Species	Application	Title
human	WB	Sci Signal; Hypoxic cancer-associated fibroblasts increase NCBP2-AS2/HIAR to promote endothelial sprouting through enhanced VEGF signaling.; Authors - Fernanda G Kugeratski
human	WB,IHC	Acta Biochim Biophys Sin (Shanghai); Angiopoietin-1 promotes triple-negative breast cancer cell proliferation by upregulating carboxypeptidase A4; Authors - Xue Liu
human,mouse	WB	Cell Death Dis; Carboxypeptidase A4 negatively regulates HGS-ETR1/2-induced pyroptosis by forming a positive feedback loop with the AKT signalling pathway; Authors - Luoling Wang