Anticorps Monoclonal anti-COX2/ Cyclooxygenase 2/ PTGS2

• Phare
• Validé par KD/KO

COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

114

CloneNo.

3G2B9

N° de cat : 66351-1-Ig

Synonymes

COX 2, COX2, COX2/ Cyclooxygenase 2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2

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Galerie de données de validation

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  WB analysis using 66351-1-Ig

  Non-treated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66351-1-Ig

  WB result of COX2/ Cyclooxygenase 2/ PTGS2 antibody (66351-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-control and sh-COX2/ Cyclooxygenase 2/ PTGS2 transfected HEK-293 cells.

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  WB analysis of RAW 264.7 using 66351-1-Ig

  Untreated and LPS-treated RAW264.7 cells were subjected to SDS PAGE followed by western blot with 66351-1-Ig (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66351-1-Ig

  Untreated and LPS-treated Hela cells were subjected to SDS PAGE followed by western blot with 66351-1-Ig (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 66351-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66351-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66351-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66351-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse lung using 66351-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (66351-1-Ig, Clone: 3G2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse lung using 66351-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (66351-1-Ig, Clone: 3G2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules HEK-293, cellules HeLa traitées au LPS, cellules RAW 264.7 traitées au LPS
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu pulmonaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 100 publications below
• IHC: See 11 publications below
• IF: See 10 publications below
• IP: See 1 publications below

Informations sur le produit

66351-1-Ig cible COX2/ Cyclooxygenase 2/ PTGS2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: COX2/ Cyclooxygenase 2/ PTGS2 Protéine recombinante Ag24721
• Nom complet: prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
• Masse moléculaire calculée: 604 aa, 68 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: BC013734
• Symbole du gène: PTGS2
• Identification du gène (NCBI): 5743
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains (PMID:14724276).

Publications

Species	Application	Title
human	WB	Cell Metab; Cooperation between COA6 and SCO2 in COX2 maturation during cytochrome c oxidase assembly links two mitochondrial cardiomyopathies.; Authors - David Pacheu-Grau
	IHC	ACS Nano; Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma; Authors - Xiao Liu
human	WB	Redox Biol; The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3; Authors - Jian Zhu
mouse	WB	J Nanobiotechnology; Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy; Authors - Junlai Wan
mouse	IHC	J Nanobiotechnology; Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression; Authors - Yunhao Wang
human	WB	ACS Appl Mater Interfaces; A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells; Authors - Xinyang Zhao