Anticorps Polyclonal de lapin anti-COX2/ Cyclooxygenase 2/ PTGS2

• Phare
• Validé par KD/KO

COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

57

Avis (1)

N° de cat : 27308-1-AP

Synonymes

COX 2, COX2, COX2/ Cyclooxygenase 2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2

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Galerie de données de validation

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  WB analysis of A549 using 27308-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 27308-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 27308-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 27308-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 27308-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 27308-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse lung using 27308-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (27308-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse lung using 27308-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (27308-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu pulmonaire humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu pulmonaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 55 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

27308-1-AP cible COX2/ Cyclooxygenase 2/ PTGS2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COX2/ Cyclooxygenase 2/ PTGS2 Protéine recombinante Ag26174
• Nom complet: prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
• Masse moléculaire calculée: 604 aa, 68 kDa
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: BC013734
• Symbole du gène: PTGS2
• Identification du gène (NCBI): 5743
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa(PMID:19656660 ). It also expresses a band of 39 kDa after unspecific cleavage(PMID:17509125 ). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains(PMID:14724276 ).

Publications

Species	Application	Title
human,mouse	WB	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
	WB	Small; M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation; Authors - Jie Sun
mouse	WB,IF	ACS Appl Mater Interfaces; Programmable Polymeric Microneedles for Combined Chemotherapy and Antioxidative Treatment of Rheumatoid Arthritis.; Authors - Chaoxiong Wu
mouse	IHC	Redox Biol; Topical hypochlorous acid (HOCl) blocks inflammatory gene expression and tumorigenic progression in UV-exposed SKH-1 high risk mouse skin.; Authors - Jana Jandova
mouse	WB,IHC	J Control Release; Combination of mitochondria impairment and inflammation blockade to combat metastasis; Authors - Xiaoli Yi
human	WB	Free Radic Biol Med; PET117 assembly factor stabilizes translation activator TACO1 thereby upregulates mitochondria-encoded cytochrome C oxidase 1 synthesis; Authors - Qiong Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ryan (Verified Customer) (02-27-2019)

Tissue was fixed in PFA with no additional antigen retrieval. Co-localisation with microglia based on known markers (not shown).

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish brain (Microglia) at 3dpf

COX2/ Cyclooxygenase 2/ PTGS2 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (Microglia) at 3dpf (Cat no:27308-1-AP)