Anticorps Monoclonal anti-COMMD5

COMMD5 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1E10G6

N° de cat : 67043-1-Ig

Synonymes

COMM domain containing 5, COMMD5, FLJ13008, HCARG, HT002

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Galerie de données de validation

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  WB analysis of human heart using 67043-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 67043-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 67043-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67043-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, cellules HEK-293, cellules MCF-7, tissu cardiaque de rat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

67043-1-Ig cible COMMD5 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: COMMD5 Protéine recombinante Ag18464
• Nom complet: COMM domain containing 5
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC003055
• Symbole du gène: COMMD5
• Identification du gène (NCBI): 28991
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COMM domain containing 5 (COMMD5, synonyms: HCARG, HT002) is a hypertension-related calcium-regulated gene. COMMD5 is negatively regulated by extracellular calcium concentration, and its basal mRNA levels were higher in hypertensive animals. Tissue distribution of COMMD5 shows a preponderance in the heart, stomach, jejunum, kidney (tubular fraction), liver, and adrenal gland (mainly in the medulla). COMMD5 mRNA is significantly more expressed in adult than in fetal organs, and its levels are decreased in tumors and cancerous cell lines. COMMD5 protein has no transmembrane domain, but contains 67% -helix content, a calcium-binding site, four putative "leucine zipper" motifs, and a nuclear receptor-binding domain. At the subcellular level, COMMD5 shows a nuclear localization.

Publications

Species	Application	Title
human	WB	Cell; Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome; Authors - Michael D Healy
human	WB	Aging (Albany NY); TMT-based quantitative proteomic analysis revealed that FBLN2 and NPR3 are involved in the early osteogenic differentiation of mesenchymal stem cells (MSCs); Authors - Jianyun Liu