Anticorps Monoclonal anti-CNPY2, MSAP

• Phare
• Validé par KD/KO

CNPY2, MSAP Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

1G8G11

N° de cat : 66173-1-Ig

Synonymes

canopy 2 homolog (zebrafish), CNPY2, CNPY2, MSAP, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9

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Galerie de données de validation

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  WB analysis using 66173-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HepG2 using 66173-1-Ig

  WB result of CNPY2, MSAP antibody (66173-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY2, MSAP transfected HepG2 cells.

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  WB analysis of human placenta using 66173-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66173-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 66173-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66173-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66173-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66173-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66173-1-Ig(CNPY2, MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-mouse IgG(H+L).

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  FC experiment of HepG2 using 66173-1-Ig

  1X10^6 HepG2 cells were stained with 0.20ug CNPY2, MSAP antibody (66173-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3, tissu hépatique de porc, tissu hépatique de rat, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66173-1-Ig cible CNPY2, MSAP dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CNPY2, MSAP Protéine recombinante Ag6363
• Nom complet: canopy 2 homolog (zebrafish)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 18-21 kDa
• Numéro d’acquisition GenBank: BC065015
• Symbole du gène: CNPY2
• Identification du gène (NCBI): 10330
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2