Anticorps Monoclonal anti-CLIC4

• Phare
• Validé par KD/KO

CLIC4 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1B7F9

N° de cat : 66343-1-Ig

Synonymes

CLIC4, CLIC4L, H1, huH1, MTCLIC, p64H1

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Galerie de données de validation

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  WB analysis of HeLa using 66343-1-Ig

  WB result of CLIC4 antibody (66343-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIC4 transfected HeLa cells.

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  WB analysis of human heart using 66343-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig heart using 66343-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig kidney using 66343-1-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 66343-1-Ig

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, cellules HeLa, tissu cardiaque de porc, tissu rénal de porc, tissu rénal de rat
• Résultats positifs en IHC: tissu cardiaque humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66343-1-Ig cible CLIC4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CLIC4 Protéine recombinante Ag2942
• Nom complet: chloride intracellular channel 4
• Masse moléculaire calculée: 253 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC012444
• Symbole du gène: CLIC4
• Identification du gène (NCBI): 25932
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIC4 is a p53- and tumor necrosis factor alpha-regulated cytoplasmic and mitochondrial protein that belongs to the CLIC family of intracellular chloride channels. CLICs have actions distinct from traditional cell membrane chloride channels, including formation of ion channels in intracellular organelles and roles in membrane trafficking, apoptosis, and cell differentiation.

Publications

Species	Application	Title
rat	WB,IF	Oxid Med Cell Longev; Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy.; Authors - Songhan Li