Anticorps Monoclonal anti-Cytokeratin 7

• Phare
• Validé par KD/KO

Cytokeratin 7 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

CloneNo.

2E1G5

Avis (1)

N° de cat : 66483-1-Ig

Synonymes

Cell and organelle markers, CK 7, CK7, Cytokeratin 7, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

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Galerie de données de validation

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  WB analysis using 66483-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66483-1-Ig (CK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66483-1-Ig

  WB result of CK7 antibody (66483-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CK7 transfected HeLa cells.

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  WB analysis of A431 using 66483-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66483-1-Ig (CK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 66483-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66483-1-Ig (CK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66483-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66483-1-Ig (CK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66483-1-Ig (CK7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66483-1-Ig (CK7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66483-1-Ig (CK7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66483-1-Ig (CK7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human prostate cancer using 66483-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Cytokeratin 7 antibody (66483-1-Ig, Clone: 2E1G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human prostate cancer using 66483-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Cytokeratin 7 antibody (66483-1-Ig, Clone: 2E1G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules BxPC-3, cellules HeLa, cellules HepG2, cellules PC-3, cellules T-47D
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu de tumeur ovarienne humain, tissu pancréatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer de la prostate humain,

Dilution recommandée

• Western Blot (WB): WB : 1:10000-1:40000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 3 publications below
• IF: See 5 publications below

Informations sur le produit

66483-1-Ig cible Cytokeratin 7 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 7 Protéine recombinante Ag7895
• Nom complet: keratin 7
• Masse moléculaire calculée: 469 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC002700
• Symbole du gène: KRT7
• Identification du gène (NCBI): 3855
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named as cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7.

Publications

Species	Application	Title
human	IHC,IF	Oxid Med Cell Longev; Deletion of ACLY Disrupts Histone Acetylation and IL-10 Secretion in Trophoblasts, Which Inhibits M2 Polarization of Macrophages: A Possible Role in Recurrent Spontaneous Abortion.; Authors - Xin Chen
human	IF	Life Sci; Cyclophilin A inhibits trophoblast migration and invasion in vitro and vivo through p38/ERK/JNK pathways and causes features of preeclampsia in mice.; Authors - Haoyue Hu
human	IHC	Placenta; MiR-187 regulates the proliferation, migration and invasion of human trophoblast cells by repressing BCL6-mediated activation of PI3K/AKT signaling.; Authors - Xin Chen
human	IF	Gene; Comprehensive analysis of placental gene-expression profiles and identification of EGFR-mediated autophagy and ferroptosis suppression in intrahepatic cholestasis of pregnancy.; Authors - Yan Fang
mouse	IF	Exp Ther Med; A novel nude mouse model for studying the pathogenesis of endometriosis.; Authors - Li-Ping Li
human	IHC	Am J Pathol; Profilin1 regulates trophoblast invasion and macrophage differentiation; Authors - Xin Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Santhosh (Verified Customer) (04-15-2019)

Human breast tumor FFPE tissue. HIER using citrate buffer. 1:200

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200

Cytokeratin 7 Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66483-1-Ig)