Anticorps Polyclonal de lapin anti-CITED1

CITED1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 26999-1-AP

Synonymes

CITED1, Melanocyte specific protein 1, MSG1

---

Galerie de données de validation

• 

  WB analysis of rat testis using 26999-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 26999-1-AP (CITED1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 26999-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 26999-1-AP (CITED1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 26999-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26999-1-AP (CITED1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 26999-1-AP

  IP Result of anti-CITED1 (IP:26999-1-AP, 4ug; Detection:26999-1-AP 1:300) with A375 cells lysate 3000ug.

• 

  IHC staining of human colon cancer using 26999-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26999-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 26999-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 26999-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 26999-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 26999-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 26999-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CITED1 antibody (26999-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293T using 26999-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human CITED1 (26999-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Applications testées

• Résultats positifs en WB: tissu testiculaire de rat, cellules A375, tissu testiculaire de souris
• Résultats positifs en IP: cellules A375
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de la thyroïde humain, tissu testiculaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,
• Résultats positifs en cytométrie: cellules HEK-293T,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

26999-1-AP cible CITED1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CITED1 Protéine recombinante Ag24643
• Nom complet: Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC004240
• Symbole du gène: CITED1
• Identification du gène (NCBI): 4435
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CITED1, also named as MSG1, belongs to the CITED family. CITED1 is a bifunctional transcriptional cofactor which is able to activate or repress transcription in association with other transcription factors. The CITED1 gene encodes a 27 kDa protein, but a 30 kDa band was also detected in the publication. The elevation of CITED1 may be directly associated with the preneoplastic phenotype (PMID: 23935526, 15272279, 15843474).

Publications

Species	Application	Title
human	WB	Science; The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation; Authors - Xin Gu

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011