Anticorps Monoclonal anti-CISD2-Specific

• Phare
• Validé par KD/KO

CISD2-Specific Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, poisson-zèbre, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

3D7A3

N° de cat : 66082-1-Ig

Synonymes

CDGSH iron sulfur domain 2, CDGSH2, CISD2, CISD2-Specific, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2

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Galerie de données de validation

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  WB analysis of HeLa using 66082-1-Ig

  WB result of CISD2 antibody (66082-1-Ig, 1:3000) with si-Control and si-CISD2 transfected HeLa cells.

• 

  WB analysis of fetal human brain using 66082-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66082-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66082-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66082-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of zebrafish using 66082-1-Ig

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 66082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 66082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 66082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 66082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 66082-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules HeLa, cellules MCF-7, cellules RAW 264.7, cellules ROS1728, tissu de poisson-zèbre
• Résultats positifs en IHC: tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66082-1-Ig cible CISD2-Specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre, rat, souris

• Réactivité: Humain, poisson-zèbre, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CDGSH iron sulfur domain 2
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: NM_001008388
• Symbole du gène: CISD2
• Identification du gène (NCBI): 493856
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. This monoclonal antibody is specific to CISD2 and does not cross-react with CISD1.

Publications

Species	Application	Title
human	WB	J Biomed Sci; Extracellular arginine availability modulates eIF2α O-GlcNAcylation and heme oxygenase 1 translation for cellular homeostasis; Authors - Yu-Wen Hung