Anticorps Monoclonal anti-CHRNA5

• Phare
• Validé par KD/KO

CHRNA5 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

3G8C11

N° de cat : 66363-1-Ig

Synonymes

CHRNA5, LNCR2, NACHRA5

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 66363-1-Ig

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig skeletal muscle using 66363-1-Ig

  pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66363-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 66363-1-Ig

  C2C12 cell were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 66363-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human bladder using 66363-1-Ig

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human bladder using 66363-1-Ig

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach using 66363-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 66363-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, cellule C2C12, cellules A549, tissu de muscle squelettique de porc, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu vésical humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 2 publications below
• IHC: See 8 publications below

Informations sur le produit

66363-1-Ig cible CHRNA5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CHRNA5 Protéine recombinante Ag4447
• Nom complet: cholinergic receptor, nicotinic, alpha 5
• Masse moléculaire calculée: 468 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC033639
• Symbole du gène: CHRNA5
• Identification du gène (NCBI): 1138
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. Variants in the CHRNA5 gene are associated with nicotine dependence and lung cancer risk.

Publications

Species	Application	Title
human,mouse	WB,IHC	Cell Mol Life Sci; Acetylcholine promotes chronic stress-induced lung adenocarcinoma progression via α5-nAChR/FHIT pathway; Authors - Yang Jiao ; KD Validated
human,mouse	IHC	J Invest Dermatol; Chrna5 is overexpressed in psoriasis patients and promotes psoriasis-like inflammation in mouse models.; Authors - Jing Wang ; KD Validated
human	IHC	Carcinogenesis; α5-nAChR associated with Ly6E modulates cell migration via TGF-β1/Smad signaling in non-small cell lung cancer.; Authors - Qian Zhang
human	IHC	Mol Carcinog; PLEK2 mediates metastasis and invasion via α5-nAChR activation in nicotine-induced lung adenocarcinoma; Authors - Qiang Li
human	IHC	Toxicology; The role of α5-nicotinic acetylcholine receptor/NLRP3 signaling pathway in lung adenocarcinoma cell proliferation and migration.; Authors - Ying Jia
human	IHC	Hum Cell; The α5-nAChR/PD-L1 axis facilitates lung adenocarcinoma cell migration and invasion.; Authors - Ping Zhu