Anticorps Polyclonal de lapin anti-CENPE

CENPE Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 28142-1-AP

Synonymes

CENP E, CENPE, Centromere protein E, centromere protein E, 312kDa, KIF10, Kinesin related protein CENPE

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Galerie de données de validation

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  WB analysis using 28142-1-AP

  HEK-293 cells lysate were subjected to SDS PAGE followed by western blot with 28142-1-AP (CENPE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28142-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28142-1-AP (CENPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 28142-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 28142-1-AP (CENPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 28142-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 28142-1-AP (CENPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

28142-1-AP cible CENPE dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CENPE Protéine recombinante Ag27230
• Nom complet: centromere protein E, 312kDa
• Masse moléculaire calculée: 316 kDa
• Poids moléculaire observé: 300-316 kDa
• Numéro d’acquisition GenBank: NM_001286734
• Symbole du gène: CENPE
• Identification du gène (NCBI): 1062
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Biochim Biophys Acta Mol Cell Res; Kinesin-7 CENP-E is essential for chromosome alignment and spindle assembly of mouse spermatocytes.; Authors - Zhen-Yu She
human	WB	Am J Transl Res; Kinesin family members KIF2C/4A/10/11/14/18B/20A/23 predict poor prognosis and promote cell proliferation in hepatocellular carcinoma.; Authors - Xishan Li
human	IHC	PLoS One; CENPE expression is associated with its DNA methylation status in esophageal adenocarcinoma and independently predicts unfavorable overall survival.; Authors - Xueqiang Zhu
mouse	WB	Cell Mol Life Sci; Global phosphoproteomic analysis identified key kinases regulating male meiosis in mouse; Authors - Haojie Li
human	IHC	Transl Oncol; A pan-cancer landscape of centromere proteins in tumorigenesis and anticancer drug sensitivity; Authors - Yuan-Xiang Shi