Anticorps Polyclonal de lapin anti-CEBPA

CEBPA Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 29388-1-AP

Synonymes

C/EBP alpha, CEBP, CEBPA

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Galerie de données de validation

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  WB analysis using 29388-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29388-1-AP (CEBPA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29388-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29388-1-AP (CEBPA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of THP-1 using 29388-1-AP

  IP result of anti-CEBPA(IP:29388-1-AP, 4ug; Detection:29388-1-AP 1:4000) with THP-1 cells lysate 1520 ug.

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Applications testées

• Résultats positifs en WB: cellules BGC-823, cellules HL-60, cellules K-562, cellules L02, cellules LNCaP, cellules THP-1, cellules U-937
• Résultats positifs en IP: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

29388-1-AP cible CEBPA dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEBPA Protéine recombinante Ag29947
• Nom complet: CCAAT/enhancer binding protein (C/EBP), alpha
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: NM_004364
• Symbole du gène: CEBPA
• Identification du gène (NCBI): 1050
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CEBPA and its isoforms play important roles in lineage determination and gene activation in a variety of cell types by activating transcription from lineage-specific promoters. CEBPA is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. In hematopoiesis, C/EBPa is a key factor in driving the development of myeloid cells interacting with a variety of factors, including c-Myc, PU.1, and microRNAs. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin which plays an important role in body weight homeostasis. CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Several pathways have been implicated as the means by which CEBPA mediates cell cycle arrest and proliferation, including p21, cyclin-dependent kinases and the E2F complex via c-Myc. The calcualted molecular weight of CEBPA is 38 kDa, but modified CEBPA is about 42 kDa (PMID: 19623175).