Anticorps Monoclonal anti-CDX2

• Phare
• Validé par KD/KO

CDX2 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

CloneNo.

5A4E6

N° de cat : 60243-1-Ig

Synonymes

caudal type homeobox 2, Caudal type homeobox protein 2, CDX 3, CDX2, CDX-2, CDX3, Homeobox protein CDX 2

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Galerie de données de validation

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  WB analysis of COLO 320 using 60243-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SW 1990 using 60243-1-Ig

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig colon using 60243-1-Ig

  pig colon tissue were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 60243-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of Caco-2 using 60243-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (60243-1-Ig, Clone: 5A4E6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HT-29, cellules SW-1990, tissu de côlon de porc
• Résultats positifs en IF: cellules Caco-2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Informations sur le produit

60243-1-Ig cible CDX2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDX2 Protéine recombinante Ag17644
• Nom complet: caudal type homeobox 2
• Masse moléculaire calculée: 313 aa, 34 kDa
• Poids moléculaire observé: 33-40 kDa
• Numéro d’acquisition GenBank: BC014461
• Symbole du gène: CDX2
• Identification du gène (NCBI): 1045
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.

Publications

Species	Application	Title
	WB	Aging (Albany NY); The role of CDX2 in renal tubular lesions during diabetic kidney disease.; Authors - Huiming Liu ; KD Validated
human	WB,IF	Mediators Inflamm; Vitronectin Destroyed Intestinal Epithelial Cell Differentiation through Activation of PDE4-Mediated Ferroptosis in Inflammatory Bowel Disease; Authors - Wenxu Pan
	IF	J Reprod Immunol; Poly(I:C) exposure during in vitro fertilization disrupts first cleavage of mouse embryos and subsequent blastocyst development.; Authors - Zhicheng Wang
human	WB	Chem Biol Interact; 6-Formylindolo(3,2-b)carbazole induced aryl hydrocarbon receptor activation prevents intestinal barrier dysfunction through regulation of claudin-2 expression.; Authors - Yuanhang Ma ; KD Validated
	IHC	World J Gastrointest Oncol; Xiaojianzhong decoction prevents gastric precancerous lesions in rats by inhibiting autophagy and glycolysis in gastric mucosal cells; Authors - Jia-Xiang Zhang
	IF	Mol Reprod Dev; Ratio of the zygote cytoplasm to the paternal genome affects the reprogramming and developmental efficiency of androgenetic embryos.; Authors - Chen Liao