Anticorps Monoclonal anti-CDK1

CDK1 Monoclonal Antibody for FC (Intra), WB

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

bovin, Humain

Applications

WB, FC (Intra)

Conjugaison

Non conjugué

Publications

1

CloneNo.

POH-1

N° de cat : 65182-1-Ig

Synonymes

CDC2, CDC28A, CDK1, Cell division protein kinase 1, Cyclin dependent kinase 1, DKFZp686L20222, p34 protein kinase

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Galerie de données de validation

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  WB analysis using 65182-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 65182-1-Ig (CDK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 65182-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 1 ug Anti-Human CDK1 (65182-1-Ig, Clone:POH-1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 1 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules K-562
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

65182-1-Ig cible CDK1 dans les applications de WB, FC (Intra) et montre une réactivité avec des échantillons bovin, Humain

• Réactivité: bovin, Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine Cdk1 humaine pleine longueur exprimée par voie bactérienne
• Nom complet: cell division cycle 2, G1 to S and G2 to M
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: BC014563
• Symbole du gène: CDC2
• Identification du gène (NCBI): 983
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par chromatographie d'affinité par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,1 %.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.

Publications

Species	Application	Title
human	WB	Cancer Genomics Proteomics; Depletion of DNTTIP2 Induces Cell Cycle Arrest in Pancreatic Cancer Cells; Authors - Masato Yoshizawa