Anticorps Polyclonal de lapin anti-CDC25A

• Phare
• Validé par KD/KO

CDC25A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

27

N° de cat : 55031-1-AP

Synonymes

CDC25A, CDC25A2, M phase inducer phosphatase 1

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Galerie de données de validation

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  WB analysis of HepG2 using 55031-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 55031-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 55031-1-AP

  IP Result of anti-CDC25A (IP:55031-1-AP, 4ug; Detection:55031-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IHC staining of human breast cancer using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human breast cancer using 55031-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 55031-1-AP (CDC25A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du sein humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 26 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

55031-1-AP cible CDC25A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cell division cycle 25 homolog A (S. pombe)
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_001789
• Symbole du gène: CDC25A
• Identification du gène (NCBI): 993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: NaHCO3 0,1 M, glycine 0,1 M, azoture de sodium à 0,02 % et glycérol à 50 %, pH 7,3.
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.

Publications

Species	Application	Title
human	WB	Nat Commun; The REGγ inhibitor NIP30 increases sensitivity to chemotherapy in p53-deficient tumor cells.; Authors - Xiao Gao
human	WB	Nat Commun; Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.; Authors - Jiliang Xia
human	WB	Cancer Res; YTHDF1 promotes gastric carcinogenesis by controlling translation of FZD7.; Authors - Jingnan Pi
human	WB,IHC,IF	Cell Death Differ; Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer.; Authors - Soumyadip Das
mouse	WB	Carbohydr Polym; Sulfate oligosaccharide of Gracilaria lemaneiformis modulates type 1 immunity by restraining T cell activation.; Authors - Qingmei Liu
human	WB	Oncogene; PTBP3 splicing factor promotes hepatocellular carcinoma by destroying the splicing balance of NEAT1 and pre-miR-612.; Authors - Xisheng Yang