Anticorps Monoclonal anti-CD98/SLC3A2

CD98/SLC3A2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

2B10F5

N° de cat : 66883-1-Ig

Synonymes

4F2, 4F2 heavy chain antigen, 4F2HC, 4T2HC, CD98, CD98/SLC3A2, CD98HC, MDU1, NACAE, SLC3A2

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Galerie de données de validation

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  WB analysis using 66883-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  IHC staining of human renal cell carcinoma using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66883-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66883-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CD98/SLC3A2 antibody (66883-1-Ig, Clone: 2B10F5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 66883-1-Ig

  1X10^6 HeLa cells were stained with 0.20ug CD98/SLC3A2 antibody (66883-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Caco-2, cellules COLO 320, cellules HepG2, cellules HT-29, cellules Jurkat, cellules MCF-7
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu de cancer du col de l'utérus humain, tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

66883-1-Ig cible CD98/SLC3A2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD98/SLC3A2 Protéine recombinante Ag7301
• Nom complet: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 85-94 kDa, 120-130 kDa
• Numéro d’acquisition GenBank: BC001061
• Symbole du gène: SLC3A2
• Identification du gène (NCBI): 6520
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; Homosalate boosts the release of tumour-derived extracellular vesicles with protection against anchorage-loss property.; Authors - Eleonora Grisard
human	WB	Nat Commun; Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9.; Authors - Mathilde Mathieu
human	WB	Cancer Cell Int; Matrix stiffness-dependent STEAP3 coordinated with PD-L2 identify tumor responding to sorafenib treatment in hepatocellular carcinoma; Authors - Shunxi Wang
human	WB,IF	Phytomedicine; Identification of the target protein and molecular mechanism of honokiol in anti-inflammatory action; Authors - Xiaoying Cai
human	WB	Oncol Lett; Hsa_circ_0050900 affects ferroptosis in intrahepatic cholangiocarcinoma cells by targeting hsa‑miR-605‑3p to regulate SLC3A2; Authors - Xiangtian Shi