Anticorps Polyclonal de lapin anti-CD90 / Thy1

CD90 / Thy1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 27178-1-AP

Synonymes

CD90, CD90 / Thy1, CDw90, Thy 1 antigen, Thy 1 cell surface antigen, Thy 1 membrane glycoprotein, THY1

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Galerie de données de validation

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  WB analysis using 27178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cérébral de souris, tissu de cancer du poumon humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 4 publications below

Informations sur le produit

27178-1-AP cible CD90 / Thy1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD90 / Thy1 Protéine recombinante Ag25603
• Nom complet: Thy-1 cell surface antigen
• Masse moléculaire calculée: 161 aa, 18 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC065559
• Symbole du gène: THY1
• Identification du gène (NCBI): 7070
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.

Publications

Species	Application	Title
rat	IF	Neurogastroenterol Motil; Construction and identification of an immortalized rat intestinal smooth muscle cell line.; Authors - Huiling Chen
rat	IF	ACS Nano; Micronano Titanium Accelerates Mesenchymal Stem Cells Aging through the Activation of Senescence-Associated Secretory Phenotype; Authors - Xuan Li
mouse	IF	Mol Immunol; Inhibition of cGAS-STING pathway by stress granules after activation of M2 macrophages by human mesenchymal stem cells against drug induced liver injury; Authors - Yao Wang
human	IF	Stem Cells; Nicotinamide riboside modulates HIF-1 signaling to maintain and enhance odontoblastic differentiation in human dental pulp stem cells; Authors - Peimeng Zhan